antibody, anti-rad-51 (rabbit polyclonal) cat Search Results


rabbit anti-rad51  (BioAcademia)
90
BioAcademia rabbit anti-rad51
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Rabbit Anti Rad51, supplied by BioAcademia, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/rad51+antibody/pmc10684888-285-41-44
Average 90 stars, based on 1 article reviews
rabbit anti-rad51 - by Bioz Stars, 2026-10
90/100 stars
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anti-rad-51 antibodies  (Strategic Diagnostics Inc)
90
Strategic Diagnostics Inc anti-rad-51 antibodies
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Anti Rad 51 Antibodies, supplied by Strategic Diagnostics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/rabbit+anti+rad51+antibodies/pmc02908675-192-0-12
Average 90 stars, based on 1 article reviews
anti-rad-51 antibodies - by Bioz Stars, 2026-10
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polyclonal rabbit anti rad51  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology polyclonal rabbit anti rad51
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Polyclonal Rabbit Anti Rad51, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/Rad51+Antibody/pmc08215854-137-32-36
Average 96 stars, based on 1 article reviews
polyclonal rabbit anti rad51 - by Bioz Stars, 2026-10
96/100 stars
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rabbit anti rad51  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit anti rad51
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Rabbit Anti Rad51, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/BRCA1+Antibody/ppr0551069-112-51-53
Average 96 stars, based on 1 article reviews
rabbit anti rad51 - by Bioz Stars, 2026-10
96/100 stars
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rabbit anti-rad-51  (GenScript corporation)
90
GenScript corporation rabbit anti-rad-51
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Rabbit Anti Rad 51, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/rabbit+anti+rad+51/pmc12135186-26-7-9
Average 90 stars, based on 1 article reviews
rabbit anti-rad-51 - by Bioz Stars, 2026-10
90/100 stars
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anti rad51  (Danaher Inc)
99
Danaher Inc anti rad51
a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified <t>RAD51</t> and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.
Anti Rad51, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/Anti-Cytochrome+C+antibody/pmc08345099-34-7-8
Average 99 stars, based on 1 article reviews
anti rad51 - by Bioz Stars, 2026-10
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anti rad51 rabbit polyclonal antibody  (Proteintech)
96
Proteintech anti rad51 rabbit polyclonal antibody
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Anti Rad51 Rabbit Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/RAD51+Antibody/pmc05666564-65-53-57
Average 96 stars, based on 1 article reviews
anti rad51 rabbit polyclonal antibody - by Bioz Stars, 2026-10
96/100 stars
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rabbit anti rad 51  (Novus Biologicals)
93
Novus Biologicals rabbit anti rad 51
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Rabbit Anti Rad 51, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/Rad51+Antibody+(5S9B0)/pm37058535-403-39-42
Average 93 stars, based on 1 article reviews
rabbit anti rad 51 - by Bioz Stars, 2026-10
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mouse monoclonal anti rad51  (Danaher Inc)
99
Danaher Inc mouse monoclonal anti rad51
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Mouse Monoclonal Anti Rad51, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/mouse+monoclonal+Anti-SOX2+antibody/pmc02483523-181-19-23
Average 99 stars, based on 1 article reviews
mouse monoclonal anti rad51 - by Bioz Stars, 2026-10
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rabbit anti rad51  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti rad51
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Rabbit Anti Rad51, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/Rad51+Rabbit+mAb/pmc11186636-345-12-14
Average 96 stars, based on 1 article reviews
rabbit anti rad51 - by Bioz Stars, 2026-10
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rabbit anti rad 51  (ABclonal Biotechnology)
94
ABclonal Biotechnology rabbit anti rad 51
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Rabbit Anti Rad 51, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/Rad51+Rabbit+pAb/pmc11077092-74-50-55
Average 94 stars, based on 1 article reviews
rabbit anti rad 51 - by Bioz Stars, 2026-10
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polyclonal anti-rad51 rabbit ab abe257  (Merck KGaA)
90
Merck KGaA polyclonal anti-rad51 rabbit ab abe257
IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and <t>RAD51</t> knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.
Polyclonal Anti Rad51 Rabbit Ab Abe257, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/antibody%2C+anti-rad-51+(rabbit+polyclonal)+cat/anti+rad51/pm36901779-215-8-16
Average 90 stars, based on 1 article reviews
polyclonal anti-rad51 rabbit ab abe257 - by Bioz Stars, 2026-10
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Image Search Results


a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified RAD51 and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: Nuclear actin polymerization rapidly mediates replication fork remodeling upon stress by limiting PrimPol activity

doi: 10.1038/s41467-023-43183-5

Figure Lengend Snippet: a iPOND analysis of HEK293T cells after indicated treatments (100 nM CPT, 1 h; 100 nM LatB, 10 min prior to CPT). See Supplementary Fig. for details. Proteins associated with nascent DNA were isolated by iPOND and detected with the indicated antibodies. In the control (Ctrl) experiment, the click reaction is performed using DMSO instead of biotin azide. Same representative experiment as in Fig. . b Graph-bar depicts mean and SD of quantified RAD51 and SMARCAL1 levels at nascent DNA from three independent iPOND experiments (black dots). Values are normalized to H3 and represented as fold change over the NT sample. Statistical analysis: one-tailed t-test with Welch’s correction. c , d Electron micrographs of representative replication forks from U2OS cells: parental (P) and daughter (D) duplexes. d White arrow indicates the regressed arm (R); the four-way junction at the reversed fork is in the inset. Scale bar = 200 nm, 40 nm in the inset. e Frequency of reversed replication forks isolated from U2OS cells upon optional treatment with 100 nM CPT for 1 h. 100 nM LatB or Swi were added 10 min before CPT and retained during the genotoxic treatment. Total number of molecules analyzed per condition in brackets. f Frequency of reversed replication forks isolated from RPE-1 cells after 24 h doxycycline-induction of either BFP-NLS or NLS-BFP-Actin R62D and optional treatment with CPT (100 nM, 1 h) or ETP (20 nM, 1 h). Total number of molecules analyzed per condition in brackets. e , f Bar graphs depict mean ± SD from three independent EM experiments (red and blue dots, respectively). Statistical analysis: ordinary one-way ANOVA. Source data are provided as a Source Data file.

Article Snippet: Proteins were resolved by electrophoresis using NuPAGE Novex 4–20% Bis-Tris gels and detected by Western blotting with the indicated antibodies: rabbit anti-Histone H3 (1:10000, abcam; ab1791), mouse anti-Pcna (1:2000, Santa-Cruz; sc-56), mouse anti-Actin (1:1000, Abcam; A5441), rabbit anti-Smarcal1 (1:1000, abcam; ab2559972), rabbit anti-Rad51 (1:1000, BioAcademia; 70-001).

Techniques: Isolation, One-tailed Test

IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and RAD51 knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.

Journal: International Journal of Medical Sciences

Article Title: IER5 is involved in DNA Double-Strand Breaks Repair in Association with PAPR1 in Hela Cells

doi: 10.7150/ijms.21510

Figure Lengend Snippet: IER5 regulates NHEJ-mediated DSB repair. a. Western blot indicating IER5 and 53BP1 knockdown by specific siRNAs. b. FACS analyses of NHEJ assay in Hela cells treated with IER5 and 53BP1 siRNAs. c. Quantification of the NHEJ assay. d. Western blot indicating IER5 and RAD51 knockdown in Hela cells upon treatment with specific siRNAs. e. Efficiency of HR, as analyzed by FACS. f. Quantification of the HR assay.

Article Snippet: Antibodies used in this study: anti-IER5 goat polyclonal antibody (Abcam), anti-IER5 rabbit polyclonal antibody (Santa Cruz), anti-GAPDH mouse monoclonal antibody (Zhong Shan Jin Qiao), anti-PARP1 rabbit monoclonal antibody (Santa Cruz), anti-Ku70 mouse monoclonal antibody (Abcam), anti-Ku80 rabbit monoclonal antibody (Santa Cruz), anti-FLAG M2 mouse monoclonal antibody (Sigma), anti-53BP1 rabbit polyclonal antibody (Santa Cruz), anti-RAD51 rabbit polyclonal antibody (Proteintech), anti-γH2AX mouse monoclonal antibody (Millipore), anti-Ubiquitinylated proteins mouse monoclonal antibody (Millipore), and anti-PADPR mouse monoclonal antibody (Abcam).

Techniques: Western Blot, Knockdown, NHEJ Assay